Anti-RPS12 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P25398

Core Product Specifications and Parameters

Biological Background: RPS12 Function and Localization

• RPS12, also known as 40S ribosomal protein S12 (eS12), is a core component of the small ribosomal subunit (40S).
• It participates in the assembly of the small subunit (SSU) processome, the earliest precursor of the small eukaryotic ribosomal subunit, in the nucleolus. During this process, RPS12 collaborates with ribosome biogenesis factors and RNA chaperones to facilitate pre-rRNA folding, modification, and cleavage (PubMed:34516797). Related references: PMID:34516797
• As a subunit of the mature 40S ribosomal complex, RPS12 contributes to the structural integrity and function of the ribosome during protein translation (By similarity).
• The protein localizes to the cytoplasm, nucleus, and nucleolus, reflecting its dual roles in ribosome biogenesis and mature ribosomal function.
• Post-translational modifications include acetylation, as evidenced by proteomic studies, and it has been directly sequenced at the protein level.
• Keywords associated with RPS12 include ribonucleoprotein, ribosomal protein, acetylation, and reference proteome, underscoring its fundamental role in the translation machinery.

Experimental Guidance and Technical Tips

• The immunogen covers full-length human RPS12 (aa 1-132), suggesting that the antibody may recognize endogenous RPS12 across human, mouse, and rat samples. Validate specificity using knockdown or knockout controls if needed.
• For Western blotting, the predicted molecular weight of RPS12 is ~15 kDa. Use high-percentage SDS-PAGE gels (e.g., 12–15%) to achieve optimal separation of low-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry, anticipate cytoplasmic and nucleolar staining. Employ fixation and permeabilization protocols that preserve nuclear architecture for accurate localization.
• For ELISA applications, the antibody can serve as a capture or detection reagent. Empirically optimize coating concentrations and detection conditions for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-RPS12 polyclonal antibody is a vital tool for investigating ribosome biogenesis, nucleolar biology, and translation regulation. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to accelerate your IVD development and research programs.