Anti-RPS10 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P46783

Core Product Specifications and Parameters

Biological Background: RPS10 Function and Localization

• RPS10 encodes the 40S ribosomal protein S10 (also known as eS10), a core component of the small ribosomal subunit.
• As part of the ribosome, RPS10 participates in the translation of mRNA into protein within the cell. Related references: PMID:23636399
• The protein localizes primarily to the cytoplasm, where ribosomes function, but is also found in the nucleus and nucleolus, reflecting its assembly into ribosomal subunits.
• RPS10 undergoes various post-translational modifications, including phosphorylation and methylation, which may regulate ribosome function or assembly.
• It is modified by ubiquitin and ISG15 (Ubl conjugation), suggesting roles in protein turnover or stress responses.
• Mutations in RPS10 are associated with Diamond-Blackfan anemia, a congenital erythroid aplasia, highlighting its importance in ribosome biogenesis.
• RPS10 belongs to the universal ribosomal protein uS10 family, conserved across prokaryotes and eukaryotes.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the full-length human RPS10 (aa 1-165), which suggests that the antibody may recognize the native protein conformation.
• For Western blotting, a band of approximately 19 kDa is expected; consider using 20–30 µg of total protein lysate from human, mouse, or rat cells.
• For immunohistochemistry (IHC-P), optimization of antigen retrieval (heat-induced, pH 6 or 9) is recommended, with a suggested primary antibody dilution range of 1:50–1:200.
• For immunofluorescence (IF/ICC), fixation with 4% paraformaldehyde and permeabilization with Triton X-100 may be suitable; validate in your specific cell system.
• The antibody is reported to cross-react with mouse and rat, but always verify specificity in your experimental model.
• In immunoprecipitation (IP), the polyclonal nature may provide robust capture; pre-clearing with protein A/G beads is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a complete range of IVD raw materials and technical support, streamlining the transition from concept to clinic. This anti-RPS10 antibody supports research into ribosome biology, protein synthesis, and associated disorders such as Diamond-Blackfan anemia. Explore our portfolio for validated antibody pairs, optimized recombinant proteins, bulk ancillary reagents, and custom sourcing solutions tailored to your IVD development needs.