Anti-RPS10 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P46783

Core Product Specifications and Parameters

Biological Background: RPS10 Function and Localization

• RPS10 encodes the 40S ribosomal protein S10 (eS10), a core component of the small ribosomal subunit, essential for ribosome biogenesis and mRNA translation.
• The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Related references: PMID:23636399
• RPS10 localizes to the cytoplasm and nucleolus, reflecting its role in ribosome assembly and export.
• As a ribosomal protein, eS10 undergoes various post-translational modifications, including phosphorylation, methylation, and conjugation by ubiquitin-like proteins, as indicated by proteomics identification and direct protein sequencing.
• Mutations in RPS10 are associated with Diamond-Blackfan anemia, a congenital disorder characterized by defective erythropoiesis and birth anomalies.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-165 of human RPS10, which corresponds to the full-length isoform 1. For Western blot, a band at approximately 19 kDa is expected; consider validating with positive control lysates from human, mouse, or rat cell lines.
• Given the polyclonal nature, this antibody may be suitable for IHC-P and IF/ICC; however, fixation and permeabilization conditions should be optimized for each sample type. Nuclear and cytoplasmic staining patterns should be confirmed.
• ELISA performance may vary depending on the coating antigen; we recommend titrating the antibody against the immunogen or native RPS10 protein to establish optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research teams global access to high-quality IVD raw materials, including validated antibodies like this anti-RPS10 polyclonal antibody for ribosomal protein studies. Our portfolio spans optimized monoclonal/polyclonal antibodies, pre-tested antibody pairs, and bulk ancillary reagents, supported by technical consultation to streamline your development from concept to clinic. For rare-target or custom sourcing needs in the ribosome biogenesis and Diamond-Blackfan anemia research areas, contact our specialist team to discuss procurement solutions.