Anti-RPRD1A Polyclonal Antibody for WB, ELISA - Q96P16

Core Product Specifications and Parameters

Biological Background: RPRD1A Function and Localization

• RPRD1A (Regulation of nuclear pre-mRNA domain-containing protein 1A), also known as P15RS or p15INK4B-related protein, is encoded by the RPRD1A gene in humans.
• It interacts with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A) and participates in CTD dephosphorylation via RPAP2.
• RPRD1A may function as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1), implicating it in cell cycle control.
• The protein localizes to the nucleus, consistent with its role in transcription regulation.
• Post-translational modifications include acetylation and phosphorylation, and the protein undergoes alternative splicing.
• Additional keywords from UniProt include a coiled-coil domain, 3D-structure, and direct protein sequencing evidence.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 47-276 of human RPRD1A; thus, the antibody is expected to recognize epitopes within this central region of the native protein.
• For Western blot, a band migrating at approximately 36 kDa is anticipated, consistent with both the supplier’s listed molecular weight and the calculated mass from UniProt. Validate detection using positive control lysates from human, mouse, or rat tissues or cell lines.
• For ELISA applications, the recombinant fusion protein used as immunogen can serve as a coating antigen to assess antibody titer and specificity.
• Consider standard blocking and washing conditions; optimize primary antibody dilution (start with 1:500–1:2000 for WB) based on signal-to-noise ratio in the target sample.
• Cross-reactivity with mouse and rat orthologs has been reported; verify sequence homology and perform appropriate tests if using these species in models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material procurement, supporting diagnostic manufacturers and research laboratories from concept to clinic. For transcription and cell cycle research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets. Our team is ready to assist with the technical and logistical aspects of integrating RPRD1A reagents into your assay development or research workflows.