Anti-RPP40 Polyclonal Antibody for WB, ELISA - O75818

Core Product Specifications and Parameters

Biological Background: RPP40 Function and Localization

• RPP40 (Ribonuclease P protein subunit p40) is a subunit of the RNase P complex, responsible for generating mature tRNA molecules by cleaving their 5′-ends. Related references: PMID:30454648, PMID:9630247
• RPP40 is also a component of the mitochondrial RNA processing (MRP) ribonuclease complex, which cleaves pre-rRNA sequences during ribosomal RNA maturation. Related references: PMID:28115465
• The protein localizes primarily to the nucleolus, a subnuclear compartment dedicated to ribosome biogenesis and RNA processing.
• Functional annotations include RNA-binding activity and involvement in both tRNA and rRNA processing pathways.
• The RPP40 gene undergoes alternative splicing, producing multiple isoforms; the protein has been directly sequenced and identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 130‑210) covers a region conserved across human, mouse, and rat, consistent with the observed cross‑reactivity; for other species, perform sequence alignment to verify expected reactivity.
• This polyclonal antibody is recommended for western blot (WB) and ELISA applications; optimal working dilutions and conditions should be titrated in the end-user’s assay system.
• In WB, a band near 42 kDa (supplier-reported) or ~41.8 kDa (UniProt theoretical weight) is expected; additional bands may appear due to splice variants or post‑translational modifications—validate band identity as necessary.
• For detection of nuclear/nucleolar targets, preparing enriched nuclear lysates may improve signal and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support diagnostic development. For research on ribonuclease complexes, tRNA processing, or nucleolar biology, CamelBio facilitates access to high-quality reagents and rare‑target raw materials—bridging the gap from initial concept to clinical assay production.