Anti-RPP38 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P78345

Core Product Specifications and Parameters

Biological Background: RPP38 Function and Localization

• RPP38 is a protein subunit of ribonuclease P, a ribonucleoprotein complex essential for the maturation of tRNA molecules by cleaving their 5'-ends. Related references: PMID:10444065 PMID:30454648 PMID:9037013
• RPP38 is also a component of the MRP ribonuclease complex, which is involved in pre-rRNA processing. Related references: PMID:28115465
• RPP38 localizes predominantly to the nucleolus within the nucleus, consistent with its function in rRNA and tRNA processing.
• Post-translational modifications of RPP38, including acetylation and phosphorylation, have been identified by proteomics studies, suggesting regulatory roles.
• RPP38 possesses RNA-binding capability, facilitating its incorporation into ribonucleoprotein complexes.

Experimental Guidance and Technical Tips

• For Western blotting, consider using this antibody to detect endogenous RPP38 at an expected molecular weight of approximately 32 kDa.
• For immunohistochemistry, validate the antibody in the appropriate formalin-fixed paraffin-embedded tissue sections; the antibody is suitable for IHC-P.
• In immunofluorescence, co-staining with nucleolar markers may help confirm the subcellular localization of RPP38.
• ELISA may be employed for quantitative detection, but optimal dilution and conditions should be determined by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw materials, providing validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support your ribonuclease P and RNA processing research. We also specialize in sourcing rare-target raw materials, streamlining your transition from discovery to clinical application.