Anti-RPP30 Rabbit pAb for WB, IHC-P, ELISA - P78346

Core Product Specifications and Parameters

Biological Background: RPP30 Function and Localization

• RPP30 (also known as RNase P subunit 2) is a protein subunit of ribonuclease P (RNase P), a ribonucleoprotein complex responsible for the 5'-end maturation of precursor tRNA molecules.
  Related references: PMID:30454648, PMID:9037013, PMID:9630247
• As a component of the RNase MRP complex, RPP30 participates in pre-rRNA processing, contributing to ribosome biogenesis.
  Related references: PMID:28115465
• RPP30 localizes primarily to the nucleolus, a subnuclear structure where ribosomal RNA synthesis and ribosome assembly occur.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, which may regulate its activity or interactions.
• RPP30 possesses RNA-binding ability, essential for its role in the catalytic ribonucleoprotein complexes.
• Alternative splicing of the RPP30 gene generates multiple transcript variants, potentially yielding protein isoforms with distinct functions.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 29 kDa is expected; validate detection in lysates from RPP30-expressing cells (e.g., HeLa, HEK293) and include appropriate controls.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval and blocking steps are recommended to minimize nonspecific background; nuclear/nucleolar staining should be observed.
• For ELISA applications, titrate the antibody and confirm performance with recombinant RPP30 protein or native complex; consider pairing with a matched detection antibody.
• Cross-reactivity with mouse and rat RPP30 may allow comparative studies, but always verify through sequence alignment and experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering a comprehensive portfolio of validated antibodies, including this anti-RPP30 polyclonal antibody for RNA processing research. Whether you need bulk quantities of core reagents or assistance sourcing rare targets like ribonuclease subunits, our platform connects you with reliable supplies and technical support from concept to clinic.