Anti-RPP25L Rabbit Polyclonal Antibody for WB, ELISA - Q8N5L8

Core Product Specifications and Parameters

Biological Background: RPP25L Function and Localization

• RPP25L is a protein encoded by the RPP25L (C9orf23) gene, also known as Rpp25-like protein, and is a putative component of ribonuclease P (RNase P) and mitochondrial RNA processing (MRP) complexes.
• These complexes are essential for tRNA and rRNA processing, with RNase P involved in tRNA 5’ maturation and MRP in mitochondrial DNA replication and rRNA processing.
• RPP25L belongs to the RPP25 family of proteins and is conserved across species.
• It localizes to the nucleus, consistent with its proposed role in RNA metabolism.
• The protein has a calculated molecular weight of approximately 17.6 kDa (observed at ~18 kDa), and has been identified in proteomics studies, indicating its expression in human cells.
• Post-translational modifications and tissue-specific expression patterns have not been extensively characterized, suggesting it may be ubiquitously expressed.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-163 of human RPP25L (full-length), suggesting the antibody may recognize the native protein.
• For Western blot, consider using nuclear-enriched fractions or whole-cell lysates from human or mouse cell lines; validate the detection at ~18 kDa.
• Cross-reactivity with mouse has been reported; consider sequence alignment to confirm antigen conservation.
• In ELISA applications, the recombinant immunogen can serve as a positive control to establish assay parameters.
• Pre-absorption with the immunogen or use of negative controls (e.g., knockout cell lines) may be helpful to confirm specificity.
• Optimize antibody dilution and incubation conditions based on your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material partner, bridging the gap from concept to clinic. We supply validated antibody pairs, optimized rabbit polyclonal antibodies like this anti-RPP25L, bulk ancillary reagents, and rare-target sourcing to diagnostic manufacturers and research laboratories worldwide. Whether you need off-the-shelf products or customized sourcing for ribonuclease P/MRP complex research, CamelBio’s integrated supply chain ensures reliable, quality-driven support from discovery through clinical development.