Anti-RPP25 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BUL9

Core Product Specifications and Parameters

Biological Background: RPP25 Function and Localization

• RPP25 (Ribonuclease P protein subunit p25) is a component of the ribonuclease P (RNase P) complex, which processes precursor tRNAs by cleaving their 5'-ends. Related references: PMID:12003489, PMID:16723659, PMID:30454648
• RPP25 is also a subunit of the MRP ribonuclease complex, essential for pre-rRNA processing. Related references: PMID:28115465
• The protein localizes primarily to the nucleolus within the nucleus, consistent with its role in RNA processing.
• RPP25 is an RNA-binding protein and undergoes phosphorylation, as indicated by proteomic data.
• The protein has a calculated molecular weight of approximately 20.6 kDa and is part of the human reference proteome.

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to the full-length RPP25 protein (amino acids 1-199), which may facilitate detection of the endogenous target across standard applications such as WB, IHC-P, and ELISA.
• For Western blotting, consider using nuclear or nucleolar extracts to enrich RPP25 signal, as the protein predominantly resides in the nucleolus.
• Cross-reactivity with mouse has been observed; thus, the antibody can be used for studies in both human and mouse model systems.
• Optimal working dilutions and incubation conditions should be empirically determined for each specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supply partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research laboratories worldwide. Our RPP25 antibody supports research into tRNA and rRNA processing, nucleolar biology, and related disease mechanisms. Beyond this product, our team can assist with sourcing rare targets and custom reagent development to accelerate your workflow from concept to clinic.