Anti-RPN2 Polyclonal Antibody for WB, ELISA - P04844

Core Product Specifications and Parameters

Biological Background: RPN2 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2) Function and Localization

• RPN2, also known as ribophorin II (RPN-II) or RIBIIR, is the 63 kDa subunit of the oligosaccharyl transferase (OST) complex. Related references: PMID:31831667
• The OST complex catalyzes the cotranslational transfer of a preassembled glycan (Glc₃Man₉GlcNAc₂) from dolichol-pyrophosphate to asparagine residues in N-X-S/T consensus motifs, initiating protein N-glycosylation in the endoplasmic reticulum (ER).
• RPN2 is an integral membrane glycoprotein with a signal sequence and transmembrane domain, localizing to the ER membrane in association with the Sec61 translocon.
• The protein undergoes post-translational modifications including N-glycosylation and ubiquitination (isopeptide bond formation).
• Alternative splicing generates multiple RPN2 isoforms, potentially modulating OST complex composition and function.
• RPN2 is ubiquitously expressed across human tissues, consistent with its housekeeping role in glycoprotein biosynthesis.
• 3D structural studies have elucidated the architecture of the OST complex, providing insights into substrate recognition and catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 360-540) corresponds to the C-terminal region of human RPN2, which is highly conserved among human, mouse, and rat orthologs, supporting the observed cross-reactivity.
• For Western blot (WB), the theoretical molecular weight is 69 kDa; however, the native protein is heavily glycosylated and may migrate at a higher apparent molecular weight. Consider using a deglycosylation enzyme to confirm band identity or to resolve glycosylation heterogeneity.
• In ELISA applications, titrate the antibody and validate specificity using recombinant RPN2 protein or cell lysates expressing the target.
• This polyclonal antibody may also be explored for immunoprecipitation (IP) or immunohistochemistry (IHC), but thorough optimization in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material procurement, providing ready-to-use antibodies and bulk ancillary reagents for glycobiology and ER-related research. For the RPN2 target, we offer validated rabbit polyclonal antibodies optimized for WB and ELISA, along with options for matched detection pairs and recombinant proteins. Let CamelBio streamline your transition from concept to clinic with comprehensive technical support and supply chain solutions.