Anti-RPN1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P04843

Core Product Specifications and Parameters

Biological Background: RPN1 Function and Localization

• RPN1 encodes the dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, also known as Ribophorin I (RPN-I), a core component of the oligosaccharyl transferase (OST) complex.
• The OST complex catalyzes the initial step of N-glycosylation by transferring a preassembled glycan from dolichol-pyrophosphate to asparagine residues within Asn-X-Ser/Thr motifs on nascent polypeptides (PubMed:31831667). Related references: PMID:31831667
• RPN1 is an integral membrane protein of the endoplasmic reticulum (ER) and associates with the Sec61 translocon complex to facilitate cotranslational glycosylation.
• All OST subunits, including RPN1, are required for maximal catalytic activity, highlighting its indispensable role in protein processing.
• Subcellular localization includes the ER membrane and, additionally, melanosomes, as identified through proteomic analyses.
• Expressed ubiquitously across all tissues tested, consistent with a fundamental housekeeping function in glycoprotein biosynthesis.
• Post-translational modifications include acetylation and ubiquitination, which may regulate protein stability or interactions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 20-280, corresponding to a conserved region of human RPN1; cross-reactivity with mouse and rat is expected based on sequence homology.
• For Western blotting, the predicted molecular weight is 69 kDa, but the endogenous protein may migrate differently due to glycosylation or other modifications; consider using deglycosylation enzymes if anomalous bands are observed.
• In immunohistochemistry (IHC-P), antigen retrieval methods should be optimized, and tissues with high secretory activity (e.g., liver, pancreas) may serve as positive controls.
• Validate antibody performance in the intended application using appropriate controls, such as knockdown or knockout cell lines, to confirm specificity.
• For ELISA, pairing with a capture antibody or optimizing coating conditions may be necessary, as this polyclonal preparation contains multiple epitope reactivities.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with a complete one-stop sourcing solution for IVD raw materials, from early R&D to clinical application. For investigations into protein N-glycosylation and ER biology, we can supply validated antibodies like this anti-RPN1 polyclonal, along with ancillary reagents such as enzyme substrates, reference proteins, and matched antibody pairs. Our global network also supports the procurement of rare-target raw materials and custom antibody development, ensuring consistent, scalable supply for your assay workflows.