Anti-RPN1 Rabbit Polyclonal Antibody for WB, ELISA - P04843

Core Product Specifications and Parameters

Biological Background: RPN1 Function and Localization

• RPN1, also known as ribophorin I (RPN-I) or dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, is a core subunit of the oligosaccharyl transferase (OST) complex.
• The OST complex catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2) from dolichol-pyrophosphate to asparagine residues within Asn-X-Ser/Thr consensus motifs, the first step in protein N-glycosylation (PMID:31831667). Related references: PMID:31831667
• N-glycosylation occurs cotranslationally, and the OST complex associates with the Sec61 translocon complex at the endoplasmic reticulum (ER) membrane.
• RPN1 is an integral membrane protein localized to the ER membrane and has also been identified in melanosomes.
• It is expressed in all tissues tested, indicating a ubiquitous role in glycoprotein synthesis.
• All subunits of the OST complex are required for maximal enzymatic activity, highlighting the essential role of RPN1 in efficient N-glycosylation.
• The protein is subject to post-translational modifications including acetylation and ubiquitination, and contains a signal peptide and transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (amino acids 22-350) of human RPN1. This region is largely lumenal and expected to recognize the native protein in its ER-localized conformation.
• For Western blot, the predicted molecular weight is 68 kDa. Use reducing conditions and appropriate loading controls. Validate with positive control lysates from human, mouse, or rat cell lines (e.g., HeLa, HEK293, NIH/3T3).
• In ELISA, this antibody may be used for direct detection or capture of recombinant RPN1 protein; consider testing against the immunogen sequence or full-length protein.
• Cross-reactivity has been observed with mouse and rat RPN1 by supplier validation, but it is advisable to confirm species-specific performance in your experimental system.
• When using for detection of endogenous RPN1, note that the protein is widely expressed; however, expression levels may vary. Optimization of antibody dilution may be necessary.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio is dedicated to providing diagnostic manufacturers, laboratories, and research institutes with comprehensive IVD raw material sourcing, from concept to clinic. This anti-RPN1 polyclonal antibody supports investigations into glycoprotein biosynthesis pathways, ER biology, and disease-associated glycosylation disorders.
• Our supply portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, all designed to streamline your research and development workflow.