Anti-RPLP2 Polyclonal Antibody for IHC-P, IF/ICC, ELISA - P05387

Core Product Specifications and Parameters

Biological Background: RPLP2 Function and Localization

• RPLP2 encodes the 60S acidic ribosomal protein P2, a component of the large ribosomal subunit, also known as renal carcinoma antigen NY-REN-44.
• It plays an essential role in the elongation step of eukaryotic protein synthesis, facilitating the interaction between the ribosome and elongation factors.
• As a ribosomal protein, RPLP2 is localized in the cytoplasm where ribosome assembly and translation occur.
• The protein is subject to post‑translational modifications, including phosphorylation and acetylation, which may regulate ribosomal activity.
• It is a member of the acidic ribosomal P protein family, sharing structural homology with P0 and P1, and has been resolved in 3D structures.
• RPLP2 has been identified as an autoantigen in certain autoimmune diseases and as a carcinoma antigen (NY-REN-44), suggesting potential roles beyond translation.

Experimental Guidance and Technical Tips

• For immunohistochemistry (IHC-P), consider optimizing the primary antibody dilution and antigen retrieval conditions, as polyclonal antibodies may exhibit lot‑to‑lot variation.
• In immunofluorescence (IF/ICC), validate the expected cytoplasmic localization and confirm specificity by co‑staining with ribosomal markers.
• For ELISA, using the recombinant immunogen (amino acids 1‑115) as a coating antigen may serve as a positive control and help establish assay linearity.
• Although western blotting is not listed, confirming a single band at ~12 kDa in relevant lysates can provide additional validation for new applications.
• Given cross‑reactivity with mouse and rat, the antibody is suitable for translational studies in common model organisms; verify species‑specific performance in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For ribosomal protein research like RPLP2, we supply high‑quality antibodies and technical support to accelerate your diagnostic development from concept to clinic.