Anti-RPLP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P05386

Core Product Specifications and Parameters

Biological Background: RPLP1 Function and Localization

• RPLP1 is a component of the large (60S) ribosomal subunit, also designated as 60S acidic ribosomal protein P1.
• It plays a critical role in the elongation step of protein synthesis by participating in ribosome function.
• Belongs to the ribosomal P protein family (L12P family), which forms the stalk structure of the ribosome and interacts with elongation factors.
• As a ribosomal protein, RPLP1 localizes to the cytoplasm where it is incorporated into ribosomes; it may also shuttle to the nucleus during ribosome assembly.
• The protein is subject to multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination/ubiquitin-like conjugation, which may regulate its function and ribosome biogenesis.
• The RPLP1 gene undergoes alternative splicing, generating potential isoforms (evidenced by the UniProt keyword “Alternative splicing”).
• The predicted molecular weight of the mature protein is approximately 11.5 kDa, consistent with the observed band around 12 kDa in western blotting.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against full-length recombinant human RPLP1 (aa 1-114), suggesting broad recognition of the native protein.
• For Western blotting, use reducing SDS-PAGE conditions; expect a specific band near 12 kDa in lysates from species showing cross-reactivity (human, mouse, rat, zebrafish).
• In immunofluorescence/ICC, cell permeabilization is required for intracellular staining; validate the localization pattern against known ribosomal markers.
• For ELISA applications, the antibody can be used as a capture or detection reagent in sandwich or indirect formats; optimize coating and detection conditions for the target analyte.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical assays, and appropriate controls (e.g., blocking with immunogen) are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio excels as a comprehensive supply partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-RPLP1 reagent, and bulk ancillary reagents for ribosomal research and protein synthesis studies. Our portfolio supports diagnostic manufacturers and laboratories from target identification through assay development, with the ability to source rare-target raw materials on demand. Whether you need monoclonal antibodies, recombinant proteins, or specialized conjugates, CamelBio provides a reliable B2B bridge to accelerate your IVD product pipeline.