Anti-RPLP0 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P05388

Core Product Specifications and Parameters

Biological Background: RPLP0 Function and Localization

• RPLP0 encodes the large ribosomal subunit protein uL10, also known as 60S acidic ribosomal protein P0 and 60S ribosomal protein L10E. It is the functional ortholog of bacterial ribosomal protein L10.
• The protein undergoes alternative splicing, producing multiple transcript variants.
• uL10 localizes to both the cytoplasm and the nucleus, consistent with its roles in ribosome biogenesis and function.
• The protein is subject to post-translational modifications including phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation.
• It is a component of the ribonucleoprotein complex and has been identified through proteomics approaches and direct protein sequencing.
• The 3D structure of uL10 has been determined, facilitating structure-function studies.
• As a member of the human reference proteome, RPLP0 is well-characterized and conserved, often serving as a stable endogenous control.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant protein (amino acids 1-317), which may promote recognition of native and denatured epitopes across applications.
• For Western blotting, the predicted molecular weight is ~34 kDa (calculated from sequence: 34,274 Da); consider using a suitable gel percentage for optimal resolution.
• The antibody demonstrates cross-reactivity with mouse RPLP0, enabling its use in mouse model studies; validation in the specific sample system is recommended.
• For ELISA and IF/ICC, titrate the antibody to determine optimal working concentrations, as these applications can be sensitive to antibody purity and format.
• Because RPLP0 is a widely expressed ribosomal protein, it is often employed as a loading control; ensure consistent sample preparation to achieve reliable normalization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by offering a comprehensive portfolio of IVD raw materials, including this high-quality anti-RPLP0 polyclonal antibody. As a one-stop sourcing bridge, we provide validated antibody pairs, optimized bulk reagents, and specialized raw-material sourcing for ribosomal and housekeeping protein targets, ensuring seamless integration from early R&D to clinical applications.