Anti-RPL9 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P32969

Core Product Specifications and Parameters

Biological Background: RPL9 Function and Localization

• RPL9 is a component of the large ribosomal subunit, essential for protein synthesis. The ribosome functions as a ribonucleoprotein complex mediating mRNA translation. Related references: PMID:23636399, PMID:32669547
• Also known as uL6, RPL9 is a core constituent of the 60S ribosomal subunit in eukaryotes.
• The protein localizes to the cytoplasm, where ribosomal assembly and protein synthesis occur.
• It undergoes acetylation, a post-translational modification that may regulate its function or interactions.
• RPL9 is part of the reference proteome and has been identified in high-throughput proteomics studies.
• The predicted molecular weight is approximately 22 kDa (UniProt: 21863 Da).
• Keywords associated with RPL9 include ribonucleoprotein and ribosomal protein, underscoring its role in the translation machinery.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human RPL9 (aa 1–192), so the antibody is expected to recognize both native and denatured forms. Optimize dilution and antigen retrieval for your specific assay.
• For Western blot analysis, the predicted band size is ~22 kDa. Include a positive control (e.g., HeLa or HEK293 cell lysate) and validate band specificity in your sample system.
• In immunofluorescence, permeabilize cells to allow nuclear/cytoplasmic staining, and use appropriate fixation to preserve target epitopes.
• As a polyclonal antibody, batch-to-batch consistency may vary; consider re-validation when switching lots.
• ELISA performance depends on plate coating conditions and detection system. Titrate the antibody to establish optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research labs with a reliable supply of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This RPL9 Rabbit Polyclonal Antibody supports research in ribosomal biology and protein synthesis, backed by CamelBio's integrated sourcing capabilities. For customized quantities, alternative conjugation, or other raw-material requests, consult our team to streamline your procurement process.