Anti-RPL9 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P32969

Core Product Specifications and Parameters

Biological Background: RPL9 (60S ribosomal protein L9) Function and Localization

• RPL9, also referred to as large ribosomal subunit protein uL6, is an essential structural constituent of the large 60S ribosomal subunit.
• It plays a critical role in ribosome biogenesis and cellular protein synthesis (PubMed:23636399, PubMed:32669547). Related references: PMID:23636399 PMID:32669547
• The protein is primarily localized in the cytoplasm, consistent with its ribosomal function.
• Post-translational modifications include acetylation, and the protein structure has been resolved in 3D studies.
• As a component of the translational machinery, RPL9 is ubiquitously expressed across mammalian tissues.
• The molecular weight of the processed protein is approximately 21.9 kDa (UniProt predicted), consistent with the observed ~22 kDa band.

Experimental Guidance and Technical Tips

• The immunogen encompasses nearly the full-length human RPL9 protein (residues 1–192), enabling recognition of both native and denatured epitopes.
• For Western blotting, a band of approximately 22 kDa is expected; validate the assay with positive controls from human or mouse cell lysates.
• In immunofluorescence/immunocytochemistry, anticipate cytoplasmic staining with possible nucleolar enrichment; include appropriate fixation and permeabilization steps.
• For ELISA applications, the polyclonal nature of the antibody may enhance detection sensitivity; consider pair-testing with a matched detection antibody.
• If cross-reacting with other species, verify specificity in the target organism’s background, as the antibody reacts with human and mouse RPL9.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents for diagnostic and research applications. For ribosomal protein targets like RPL9, we support procurement of bulk quantities, custom production, and technical consultation to streamline your assay development from concept to clinical use.