Anti-RPL8 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62917

Core Product Specifications and Parameters

Biological Background: RPL8 Function and Localization

• RPL8, also known as large ribosomal subunit protein uL2 or 60S ribosomal protein L8, is a component of the large (60S) ribosomal subunit.
• The ribosome is a large ribonucleoprotein complex responsible for protein synthesis in the cell, with RPL8 contributing to its structural integrity and function.
• RPL8 is primarily localized in the cytoplasm, the site of ribosomal translation.
• As a ribosomal protein, RPL8 possesses RNA-binding and rRNA-binding domains, essential for ribosome assembly and stability.
• The protein is subject to various post-translational modifications, including hydroxylation, isopeptide bond formation, and ubiquitin-like conjugation.
• RPL8 has been extensively characterized through direct protein sequencing, proteomics identification, and 3D structural studies.

Experimental Guidance and Technical Tips

• For Western blotting, use a positive control lysate from human, mouse, or rat cells; the predicted molecular weight is 28 kDa, though actual migration may vary due to post-translational modifications.
• When performing immunohistochemistry (IHC-P) or immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization steps based on tissue type and fixation method.
• As a polyclonal antibody, it may recognize endogenous RPL8 across species; validate specificity in your experimental system using appropriate knockout or knockdown controls.
• For ELISA applications, consider pairing with a suitable detection antibody and verify the assay conditions for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for IVD raw materials, technical services, and consultation, supporting diagnostic manufacturers and research institutes from concept to clinic. This anti-RPL8 polyclonal antibody enables robust research into ribosome biology and protein synthesis, with reliable performance across multiple applications. Through CamelBio, you can efficiently source validated antibody pairs, bulk ancillary reagents, and even rare-target raw materials to accelerate your assay development and production.