Anti-RPL7A Monoclonal Antibody for WB, ELISA - P62424

Core Product Specifications and Parameters

Biological Background: RPL7A Function and Localization

• RPL7A, also known as 60S ribosomal protein L7a, eL8, SURF3, or PLA-X polypeptide, is a key protein component of the large ribosomal subunit.
• It is integral to the ribosome, the molecular machine responsible for cellular protein synthesis (translation). Related references:
  PMID:23636399
  PMID:32669547
• The human RPL7A gene belongs to the L7AE ribosomal protein family and is located in the surfeit locus, often involved in chromosomal rearrangements.
• Subcellularly, RPL7A is localized in the cytoplasm, consistent with its role in cytoplasmic translation.
• Post-translational modifications of RPL7A include acetylation, isopeptide bond formation, and ubiquitin conjugation, which may modulate its function or stability.
• Keywords associated with RPL7A include ribonucleoprotein, 3D-structure, and reference proteome, highlighting its structural and proteomic characterization.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1–165), which likely contains conserved epitopes; confirm reactivity with endogenous protein in relevant sample systems.
• For western blot (WB), consider standard denaturing and reducing SDS-PAGE conditions; the predicted band is approximately 30 kDa.
• For ELISA, the antibody can be tested as a capture or detection reagent; optimal working concentrations should be determined empirically.
• Cross-reactivity with mouse and rat RPL7A has been reported; validate in your specific assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and researchers, providing validated antibodies like this RPL7A monoclonal antibody. We offer one-stop access to antibody pairs, recombinant proteins, and ancillary reagents for ribosome biology and translation studies. From concept to clinic, CamelBio supports your development with bulk supply and rare target sourcing.