Anti-RPL4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P36578

Core Product Specifications and Parameters

Biological Background: RPL4 Function and Localization

• The RPL4 gene encodes the large ribosomal subunit protein uL4, also known as 60S ribosomal protein L4 (or L1).
• As a core ribosomal component, uL4 participates in the structure and function of the large ribosomal subunit, essential for protein biosynthesis.
• The protein localizes predominantly to the cytoplasm, reflecting its role in the ribosomal machinery.
• Post-translational modifications include acetylation, citrullination, methylation, and phosphorylation, as evidenced by proteomic studies and direct protein sequencing.
• RPL4 is part of the ribonucleoprotein complex and is subject to ubiquitin conjugation (Ubl conjugation).
• Three-dimensional structural information is available for this protein, aiding in the understanding of its ribosomal integration.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal 1–240 amino acids of human RPL4, which may cover conserved regions. When applying to tissues or cell lines, consider validating expression in your specific sample system.
• For Western blotting, the predicted molecular weight of RPL4 is ~48 kDa, but post-translational modifications may cause slight shifts. Use appropriate blocking and detection reagents.
• For immunohistochemistry (paraffin-embedded), antigen retrieval may be necessary. Titrate the antibody carefully to achieve optimal staining.
• This antibody cross-reacts with mouse and rat RPL4; confirm sequence homology if using in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD manufacturers and research laboratories developing assays around ribosomal targets like RPL4. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and access to rare-target raw materials, supporting your transition from concept to clinic with a single procurement partner. This RPL4 polyclonal antibody exemplifies our commitment to reliable tools for protein biosynthesis studies and related diagnostic applications.