Anti-RPL39 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P62891

Core Product Specifications and Parameters

Biological Background: RPL39 Function and Localization

• RPL39 encodes the 60S ribosomal protein L39, also known as large ribosomal subunit protein eL39, a conserved component of the cytoplasmic ribosome.
• As an RNA-binding component of the large ribosomal subunit, RPL39 contributes to the structural integrity and function of the ribosome, a large ribonucleoprotein complex responsible for protein synthesis in cells.
• RPL39 belongs to the eukaryotic ribosomal protein eL39 family, characterized by its small size (~6.4 kDa) and high basic amino acid content, facilitating interaction with ribosomal RNA.
• The protein localizes exclusively to the cytoplasm, where it assembles into the 60S subunit and participates in mRNA translation.
• Keywords associated with RPL39 include ribonucleoprotein, ribosomal protein, cytoplasm, and proteomics identification, underscoring its fundamental role in translation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1–51 of human RPL39 (NP_000991.1); this region is suitable for generating polyclonal antibodies with broad epitope recognition.
• For Western blot (WB), consider that RPL39 has a molecular weight of approximately 6 kDa; optimize SDS-PAGE and transfer conditions (e.g., use of 0.2 μm membrane, low molecular weight markers) to reliably detect this small protein.
• In immunofluorescence/immunocytochemistry (IF/ICC), anticipate a cytoplasmic staining pattern consistent with ribosomal localization.
• The antibody is reported to cross-react with human samples; validate in your specific experimental system, including relevant cell lines or tissue lysates.
• For ELISA, use the antibody as a capture or detection reagent in sandwich formats, ensuring proper coating concentrations and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes with comprehensive access to IVD raw materials like the RPL39 rabbit polyclonal antibody, tailored for translational and ribosomal studies. Our supply chain integrates validated antibody pairs, optimized monoclonal/polyclonal preparations, bulk ancillary reagents, and rare-target sourcing, streamlining your procurement from discovery to assay development. Partner with CamelBio to bridge concept and clinic with consistent, research-grade materials.