Anti-RPL37 Polyclonal Antibody for WB, ELISA - P61927

Core Product Specifications and Parameters

Biological Background: RPL37 Function and Localization

• RPL37 is a core component of the large ribosomal subunit, participating in the ribonucleoprotein complex responsible for cellular protein synthesis. Related references: PMID:23636399 PMID:32669547
• RPL37 belongs to the eukaryotic large ribosomal subunit proteins and is a member of the universal ribosomal protein family, featuring a zinc-finger domain that suggests metal-binding and RNA-binding capabilities.
• Subcellularly, RPL37 is localized in the cytoplasm, consistent with its role in ribosomal assembly and function.
• Post-translational modifications of RPL37 include acetylation and phosphorylation, which may regulate its function or ribosome assembly.
• The protein has a calculated molecular weight of approximately 11 kDa, consistent with the observed 11 kDa in experimental detection.
• As part of the large ribosomal subunit, RPL37 contributes to the structural integrity and translational function of the ribosome, potentially impacting cellular growth and proliferation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (amino acids 1-97) of human RPL37. For Western blotting, the recommended molecular weight is 11 kDa; use appropriate molecular weight markers and validate with a known positive control.
• Since this is a rabbit polyclonal antibody, consider optimizing dilutions for WB and ELISA based on the target abundance in your sample. Pilot experiments are recommended to determine the ideal working concentration.
• The antibody shows cross-reactivity with human, mouse, and rat, allowing detection across multiple species. Verify specificity in your particular sample system.
• For ELISA applications, evaluate direct or indirect detection formats and consider peptide blocking to confirm epitope specificity.
• Always include proper negative controls (e.g., knockout cell lines or tissue with low RPL37 expression) to ensure signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-RPL37 polyclonal antibody is part of our extensive portfolio targeting ribosomal proteins and translation research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets, streamlining your procurement process for reliable supply chains.