Anti-RPL36A Polyclonal Antibody for WB, ELISA - P83881

Core Product Specifications and Parameters

Biological Background: RPL36A (eL42) Function and Localization

• RPL36A encodes the large ribosomal subunit protein eL42, also annotated as 60S ribosomal protein L36a, 60S ribosomal protein L44, cell growth-inhibiting gene 15 protein (MIG6), and cell migration-inducing gene 6 protein.
• As a component of the 60S large ribosomal subunit, eL42 participates in ribosome assembly and is essential for cytosolic protein synthesis.
• The ribosome is a macromolecular machine responsible for translating mRNA into proteins; eL42 contributes to its structural scaffold and functional integrity.
• Subcellularly, eL42 localizes to the cytoplasm, consistent with its role in translation.
• Keywords associated with RPL36A include 3D-structure, cytoplasm, ribonucleoprotein, and ribosomal protein, underscoring its structural role in the ribosomal particle.
• The predicted molecular weight is approximately 12.4 kDa (12441 Da), with an observed band near 12 kDa on SDS-PAGE under reducing conditions.

Experimental Guidance and Technical Tips

• The immunogen—a recombinant protein spanning amino acids 37–142 of human RPL36A—targets a conserved core region and is expected to detect endogenous full-length protein in human samples.
• For Western blot, consider using whole‑cell or cytoplasmic extracts from human cell lines; a specific band around 12 kDa is anticipated.
• ELISA applications may be developed using the antibody as capture or detection reagent; validate performance with recombinant RPL36A or relevant peptide standards.
• Cross‑reactivity with other species has not been characterized; if using non‑human samples, verify specificity empirically.

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CamelBio streamlines IVD raw‑material procurement with a comprehensive portfolio of antibodies, antigens, and ancillary reagents. For ribosomal protein research, including targets like RPL36A/eL42, we offer validated polyclonal and monoclonal antibodies, recombinant proteins, and custom sourcing. Contact us to explore bulk ordering, technical consultation, or rare‑target raw‑material solutions tailored to your diagnostic development.