Anti-RPL35 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P42766

Core Product Specifications and Parameters

Biological Background: RPL35 Function and Localization

• RPL35, also known as 60S ribosomal protein L35 or large ribosomal subunit protein uL29, is a key component of the large (60S) ribosomal subunit.
• It is integral to ribosome structure and function, facilitating protein synthesis within the cell. Related references: PMID:12962325 PMID:23636399 PMID:32669547
• The protein localizes to the cytoplasm, where ribosomes are actively engaged in translating mRNA into polypeptide chains.
• RPL35 is subject to post‑translational modifications including acetylation, phosphorylation, and ubiquitin conjugation (isopeptide bond), which may regulate its activity or ribosome assembly.
• Mutations in RPL35 are associated with Diamond‑Blackfan anemia, a bone marrow failure syndrome, highlighting its clinical relevance.
• High‑resolution 3D structures of the human ribosome have revealed the positioning of uL29 within the 60S subunit.
• The protein is also identified through direct protein sequencing and mass spectrometry, confirming its expression in human tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–120 of human RPL35. Verify the epitope accessibility in your target species and sample type.
• For Western blot (WB), a band at approximately 15 kDa is expected; consider using a positive control such as HeLa or HEK‑293 cell lysate.
• In immunohistochemistry (IHC‑P), proper antigen retrieval may be required; we recommend optimizing retrieval conditions and antibody dilution.
• For immunofluorescence (IF/ICC), fixation and permeabilization protocols should be tailored to achieve clear cytoplasmic staining.
• Cross‑reactivity with mouse and rat has been confirmed; however, further validation in your model system is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with global diagnostic manufacturers and research laboratories to provide a comprehensive range of IVD raw materials. This anti‑RPL35 polyclonal antibody, targeting a core ribosomal protein, supports investigations into protein synthesis and ribosomopathies. Beyond single antibodies, CamelBio assists in sourcing validated antibody pairs, bulk ancillary reagents, and custom rare‑target raw materials, simplifying your supply chain from concept to clinic.