Anti-RPL34 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P49207

Core Product Specifications and Parameters

Biological Background: RPL34 Function and Localization

• RPL34 encodes the 60S ribosomal protein L34 (eL34), a component of the large ribosomal subunit.
• As part of the ribosome, RPL34 contributes to the essential process of protein synthesis, facilitating the translation of mRNA into polypeptide chains. Related references: PMID:12962325, PMID:23636399, PMID:25901680
• The protein localizes to the cytoplasm, cytosol, and endoplasmic reticulum, reflecting its role in both cytosolic translation and co-translational protein targeting.
• RPL34 is subject to multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), as identified by proteomics.
• The mature protein has a calculated molecular mass of 13,293 Da (UniProt), consistent with the experimentally observed ~13 kDa band.
• RPL34 is listed as a component of the human reference proteome, underscoring its well-characterized role in ribosome biology.

Experimental Guidance and Technical Tips

• For western blotting (WB), target detection at ~13 kDa in whole cell lysates from human or mouse samples can be expected. Optimize antibody dilution according to your experimental conditions.
• In immunofluorescence (IF/ICC), strong cytoplasmic staining with possible ER association may be observed; include appropriate controls and consider fixation/permeabilization protocols for ribosomal antigens.
• For ELISA applications, using recombinant RPL34 protein as a positive control is recommended to establish standard curves.
• As a polyclonal antibody, batch-to-batch variability may occur; validate performance in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material sourcing, offering comprehensive solutions from validated antibody pairs to bulk ancillary reagents. For ribosomal and translation-focused projects, we provide high-quality antibodies like this anti-RPL34 polyclonal antibody, along with access to optimized reagents and technical consulting. Whether you need rare target antibodies or scalable raw materials, CamelBio bridges the gap from concept to clinic.