Anti-RPL32 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62910

Core Product Specifications and Parameters

Biological Background: RPL32 Function and Localization

• RPL32 encodes the 60S ribosomal protein L32 (eL32), a component of the large ribosomal subunit.
• As an integral part of the ribosome, RPL32 is essential for protein synthesis (translation). Related references: PMID:23636399 PMID:32669547
• RPL32 is a cytoplasmic protein, consistent with its ribosomal localization.
• The protein is subject to multiple post-translational modifications, including phosphorylation, isopeptide bond formation, and ubiquitination (Ubl conjugation).
• RPL32 is a ribonucleoprotein that interacts with rRNA within the large subunit.
• Structural studies (3D-structure annotation) and proteomic identification confirm its role in ribosome assembly.
• The high conservation of RPL32 across mammals is reflected in the antibody's cross-reactivity with mouse and rat orthologs.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering amino acids 1-135 of human RPL32. As a polyclonal antibody, it recognizes multiple epitopes, providing robust signal in various applications.
• For Western blot, expect a band at approximately 16 kDa. Whole cell lysates from human, mouse, or rat samples are suitable.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider optimizing antigen retrieval conditions for your specific tissue or cell type.
• This antibody can be used in ELISA for detection or quantification, but appropriate controls and standard curves should be established.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated sourcing of IVD raw materials, including this anti-RPL32 antibody for ribosomal protein and translation research. Our platform offers validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support your assay development. Count on CamelBio to connect your concept to the clinic with reliable, one-stop access to critical components.