Anti-RPL31 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P62899

Core Product Specifications and Parameters

Biological Background: RPL31 Function and Localization

• Encoded by the RPL31 gene, the protein eL31 (also designated 60S ribosomal protein L31) is a core constituent of the large (60S) ribosomal subunit.
• Functions as a component of the large ribosomal subunit, facilitating mRNA translation and protein synthesis. Related references: PMID:23636399 PMID:32669547
• Subcellular localization is primarily cytoplasmic, consistent with ribosomal assembly and function.
• Undergoes post-translational modifications, including acetylation and phosphorylation, which may influence ribosome activity or stability.
• Multiple transcript variants arise via alternative splicing, potentially yielding isoforms with tissue-specific functions.
• Classified as a ribosomal protein and ribonucleoprotein, reflecting its structural and functional role in the translation machinery.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 5–105) of human RPL31; consider sequence alignment across orthologs to predict cross-reactivity.
• For Western blot (WB), expect a band near 14 kDa; validate detection in your target species and tissue lysates with appropriate controls.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution for the specific sample type and fixation method.
• ELISA applications may require coating antigen optimization and careful titration to achieve optimal signal-to-noise ratios.
• Given the conserved nature of ribosomal proteins, pre-adsorption or knockout validation is advised to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and optimized antibodies for targets like RPL31 involved in ribosome biology and translational research. Our supply network supports diagnostic manufacturers and laboratories from early-stage assay development to clinical application, with customized solutions for rare targets and challenging reagents. Reach out to explore how our one-stop model can streamline your next project.