Anti-RPL30 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62888

Core Product Specifications and Parameters

Biological Background: RPL30 Function and Localization

• RPL30 (also known as L30/eL30) is a 60S ribosomal protein encoded by the RPL30 gene in humans, serving as a key component of the large ribosomal subunit.
• As a ribosomal protein, RPL30 participates in ribosome assembly and is required for protein synthesis in the cell. Related references: PMID:23636399 PMID:32669547
• RPL30 is primarily localized in the cytoplasm, consistent with the site of ribosome function and assembly.
• The protein undergoes multiple post-translational modifications, including acetylation, phosphorylation, isopeptide bond formation, and ubiquitin conjugation, which may modulate its activity or interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human RPL30 protein (amino acids 1-115); thus the antibody may recognize both native and denatured conformations, making it suitable for applications like WB and ELISA.
• For Western blot, a broad range of dilutions should be tested due to the high abundance of RPL30 in cells; a starting dilution of 1:500–1:2000 is a common initial range for polyclonals.
• In IHC-P and IF/ICC, signal optimization may require antigen retrieval and permeabilization steps; validate using appropriate positive control tissues or cell lines.
• Given the cross-reactivity with mouse and rat, this antibody can be employed in comparative translational studies across species, but confirm cross-reactivity in the specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your procurement of high-quality IVD raw materials, from specialized polyclonal antibodies like anti-RPL30 to complete reagent panels for ribosomal research. As a dedicated B2B partner, we support diagnostic manufacturers and laboratories with validated antibody pairs, optimized bulk reagents, and custom sourcing for rare ribosomal targets—backed by end-to-end technical and consulting services from concept to clinic.