Anti-RPL3 Rabbit Polyclonal Antibody for WB, ELISA - P39023

Core Product Specifications and Parameters

Biological Background: RPL3 (Large ribosomal subunit protein uL3) Function and Localization

• RPL3 is an integral component of the 60S large ribosomal subunit, critical for the assembly and stability of the ribosome (PubMed:12962325, PubMed:23636399, PubMed:32669547). Related references: PMID:12962325 PMID:23636399 PMID:32669547
• As a ribosomal protein, RPL3 is essential for the translation process, where it contributes to the decoding of mRNA and synthesis of proteins (PubMed:12962325, PubMed:23636399, PubMed:32669547). Related references: PMID:12962325 PMID:23636399 PMID:32669547
• The RPL3 gene encodes a member of the conserved ribosomal protein uL3 family, highlighting its universal role in cellular translation machinery.
• RPL3 localizes to the nucleolus in the nucleus for ribosome biogenesis and to the cytoplasm where mature ribosomes are active.
• Also identified as HIV-1 TAR RNA-binding protein B (TARBP-B), suggesting involvement in HIV viral RNA binding.
• Post‑translational modifications such as acetylation, methylation, and phosphorylation are reported for RPL3, potentially modulating its activity.
• UniProt keywords: Acetylation, Methylation, Phosphoprotein, Ribonucleoprotein, Ubl conjugation, indicating a dynamic regulatory landscape.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 1–260 of human RPL3 (NP_000958.1); when designing epitope‑targeted assays, consider this N‑terminal region coverage.
• For Western blotting, a band of approximately 46 kDa is expected; validate in your specific cell or tissue lysates with appropriate loading controls.
• ELISA systems may require thorough optimization of coating and detection conditions; recombinant RPL3 protein can serve as a positive control.
• Cross-reactivity with mouse RPL3 is reported; verify performance in mouse‑derived samples to ensure consistent detection.
• As a polyclonal antibody, lot‑to‑lot variation is possible; re‑validate each new batch in your assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti-RPL3 antibody for research into ribosome biology and translation. Our catalog includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Streamline your development pipeline with CamelBio, from concept to clinic.