Anti-RPL27 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P61353

Core Product Specifications and Parameters

Biological Background: RPL27 Function and Localization

• RPL27, also known as 60S ribosomal protein L27 or eL27, is a core component of the large (60S) ribosomal subunit, essential for protein synthesis. Related references: PMID:12962325, PMID:23636399, PMID:25901680
• It is specifically required for the processing and maturation of ribosomal RNAs, notably the production of mature 28S and 5.8S rRNAs. Related references: PMID:25424902
• The protein localizes to the cytoplasm, with prominent distribution in the cytosol and on the rough endoplasmic reticulum, consistent with its role in translation.
• RPL27 undergoes post-translational modifications including acetylation, and has been characterized via direct protein sequencing and mass spectrometry-based proteomics.
• Mutations in the RPL27 gene are linked to Diamond-Blackfan anemia, underscoring its critical function in ribosome biogenesis and erythropoiesis.
• Encoded by the RPL27 gene on chromosome 17, the full-length human protein comprises 136 amino acids with a predicted molecular mass of approximately 15.8 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans the entire human RPL27 sequence (aa 1–136), so the antibody is expected to detect both native and denatured protein. In Western blot, a band near 16 kDa is anticipated; whole-cell lysates from human, mouse, or rat should be suitable given the reported cross-reactivity.
• As a polyclonal preparation, this antibody may offer robust signal for applications like ELISA and IF/ICC. Validation in the relevant sample system with appropriate controls (e.g., RPL27 silencing or knockout) is recommended to confirm specificity.
• Due to high evolutionary conservation, cross-reaction with other mammalian species is possible but should be experimentally verified if deviating from the tested species.
• In immunofluorescence, anticipate cytoplasmic/perinuclear staining; co-localization with an ER marker can assist in confirming subcellular distribution.

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CamelBio provides diagnostic manufacturers and research teams with streamlined access to premium IVD raw materials like this anti-RPL27 antibody. Whether your projects require validated antibody pairs, optimized polyclonals, or bulk ancillary reagents, our platform supports procurement from early-stage R&D through clinical application. Explore our ribosomal antibody portfolio to accelerate your translation and protein synthesis assays.