Anti-RPL26L1 Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - Q9UNX3

Core Product Specifications and Parameters

Biological Background: RPL26L1 Function and Localization

• RPL26L1, also known as 60S ribosomal protein L26-like 1 or ribosomal protein uL24-like (UniProt Q9UNX3), is a ribosomal protein encoded by the RPL26L1 gene (also designated RPL26P1).
• As a component of the 60S large ribosomal subunit, it is expected to participate in ribosome assembly and protein translation, fundamental to cellular homeostasis.
• It belongs to the L26 ribosomal protein family, which includes the closely related RPL26 (60S ribosomal protein L26), suggesting functional redundancy or specialized roles in ribosome biogenesis.
• Consistent with its ribosomal function, RPL26L1 primarily localizes to the cytoplasm, with likely nucleolar trafficking during subunit synthesis and maturation.
• The protein features post-translational modifications, including isopeptide bond formation and ubiquitin-like (Ubl) conjugation, as indicated by UniProt keyword annotations.
• Its expression has been verified through direct protein sequencing and proteomics identification, supporting its status as a reference proteome component in human cells.
• The mature protein has a calculated molecular weight of approximately 17 kDa (17256 Da based on UniProt data), consistent with the reported 17 kDa band in Western blot assays.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1-100), which may preferentially detect the full-length protein; consider using a blocking peptide or knockout validation to confirm specificity.
• For Western blot, expect a band around 17 kDa; optimal dilution should be determined empirically, and a positive control such as HeLa or HEK293 whole cell lysate is recommended.
• In immunofluorescence and immunohistochemistry, fixation/permeabilization conditions need optimization due to the high abundance of ribosomal proteins; titration of antibody concentration is advised to minimize background.
• Reported cross-reactivity with mouse and rat origins suggests utility for translational studies across species; however, validation in the relevant sample system is strongly encouraged.

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CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-RPL26L1 antibody. For ribosomal protein targets and translation-related research, we offer matched antibody pairs, ancillary reagents, and custom sourcing of bulk raw materials. Reach out to explore our supply capabilities and accelerate your development from concept to clinic.