Anti-RPL26 Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P61254

Core Product Specifications and Parameters

Biological Background: RPL26 Function and Localization

• RPL26 (60S ribosomal protein L26 / uL24) is a structural constituent of the large ribosomal subunit.
• As a ribosomal protein, it is essential for protein biosynthesis and ribosome assembly (PubMed:23636399, PubMed:26100019, PubMed:32669547).
  Related references:
  PMID:23636399
  PMID:26100019
  PMID:32669547
• The protein is predominantly located in the cytoplasm, the site of protein translation.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond formation), as indicated by UniProt keywords.
• RPL26 is annotated with the disease term Diamond-Blackfan anemia, a disorder of ribosomal dysfunction.
• The immunogen spans the full-length human RPL26 (amino acids 1-145), ensuring coverage of key structural domains.

Experimental Guidance and Technical Tips

• The monoclonal nature of this antibody ensures batch-to-batch consistency, which is critical for diagnostic assay development.
• For Western blot, the predicted molecular weight is 17 kDa; ensure appropriate gel percentage and transfer conditions for low-molecular-weight proteins.
• Given its reactivity with human, mouse, and rat, this antibody can be used across preclinical models, but validation in the specific tissue or cell line is recommended.
• Antigen retrieval may be necessary for IHC-P; consider heat-induced epitope retrieval in citrate buffer (pH 6.0) as a starting point.
• ELISA applications may benefit from pairing with a matched detection antibody; test direct vs. indirect formats under your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials for ribosomal research and translational biology, including validated monoclonal antibodies like this anti-RPL26 clone. We support diagnostic manufacturers and labs with cost-effective sourcing of optimized antibody pairs, bulk reagents, and rare target materials, helping streamline your assay development from concept to clinic. Whether you need custom conjugation, lyophilized formats, or technical consulting, CamelBio bridges the gap between raw material supply and commercial IVD success.