Anti-RPL24 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P83731

Core Product Specifications and Parameters

Biological Background: RPL24 Function and Localization

• RPL24 (60S ribosomal protein L24, also known as eL24) is a component of the large (60S) ribosomal subunit, essential for protein synthesis.
• The ribosome is a large ribonucleoprotein complex that translates mRNA into polypeptide chains; RPL24 contributes to its structural integrity and function.
• Alternative names include 60S ribosomal protein L30 (historical designation), reflecting its conserved role across eukaryotes.
• RPL24 localizes primarily to the cytoplasm, where ribosomes are assembled and active in translation.
• Post-translational modifications of RPL24 include acetylation, phosphorylation, and ADP-ribosylation, which may regulate ribosomal activity or interactions.
• RPL24 contains isopeptide bonds and is subject to ubiquitin conjugation (Ubl conjugation), potentially affecting its stability or function.
• The protein has been identified through direct protein sequencing and proteomics studies, confirming its expression and presence in human cells.

Experimental Guidance and Technical Tips

• For Western blot, the expected molecular weight is approximately 18 kDa; use appropriate loading controls.
• When performing immunohistochemistry (IHC-P), optimize antigen retrieval conditions for the specific tissue type.
• In immunofluorescence (IF/ICC), confirm cytoplasmic localization consistent with ribosomal distribution.
• For ELISA, titrate both antibody and antigen to determine optimal working concentrations.
• The antibody is polyclonal; consider including negative controls (e.g., pre-immune serum or blocking peptide) in initial experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibodies for translation and ribosomal protein research. Our supply capabilities encompass optimized polyclonal antibodies like this RPL24 antibody, as well as bulk ancillary reagents and rare target sourcing. Partner with CamelBio to streamline your IVD development from concept to clinic.