Anti-RPL23 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62829

Core Product Specifications and Parameters

Biological Background: RPL23 Function and Localization

• RPL23, also known as 60S ribosomal protein L23 or uL14, is a component of the large (60S) ribosomal subunit in the ribosome.
• It plays a key role in protein synthesis as part of the ribosome's peptidyl transferase center and structural framework.
• RPL23 is encoded by the RPL23 gene (Gene ID: 9349) and belongs to the universal ribosomal protein uL14 family.
• Subcellularly localized to the cytoplasm, RPL23 assembles with ribosomal RNA and other proteins to form the active ribosome.
• The protein undergoes phosphorylation, which may regulate ribosome activity or translation under various cellular conditions (Keyword: Phosphoprotein).
• As a reference proteome component, RPL23 has been identified in high-throughput proteomics studies, confirming its ubiquitous expression.
• Its molecular weight is approximately 14.9 kDa (theoretical), consistent with the observed 15 kDa band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human RPL23 (aa 1–140), suggesting potential detection of both full-length and truncated forms if present. Verify band size in your sample system.
• For Western blot (WB), a band around 15 kDa is expected. Use appropriate low molecular weight markers and transfer conditions for small proteins.
• For IHC-P and IF/ICC, consider antigen retrieval and permeabilization protocols suitable for ribosomal antigens in cytoplasm.
• While the product shows cross-reactivity with mouse and rat, validate in your specific species/tissue if not tested.
• For ELISA, coat with the recombinant protein or cell lysate, and pair with a matched detection antibody if developing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop IVD raw-material sourcing partner, CamelBio provides high-quality antibodies like this anti-RPL23 polyclonal antibody for ribosome biology and protein synthesis research. We also offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your diagnostic development. Let CamelBio bridge your concept to clinic with comprehensive material supply and technical support.