Anti-RPL21 Rabbit Polyclonal Antibody - P46778

Core Product Specifications and Parameters

Biological Background: RPL21 Function and Localization

• RPL21 encodes the 60S ribosomal protein L21 (also designated eL21), which is a structural component of the large ribosomal subunit and participates in protein synthesis. Related references: PMID:12962325 PMID:23636399 PMID:25901680
• The protein is also known as HYPT12 due to its association with hypotrichosis; mutations are linked to hair loss disorders, suggesting tissue-specific roles.
• RPL21 primarily localizes to the cytoplasm, where it is found in the cytosol and associated with the endoplasmic reticulum.
• Mature RPL21 has a calculated molecular weight of approximately 18.6 kDa, running at ~19 kDa on SDS-PAGE.
• As a ribosomal protein, RPL21 is subject to various post-translational modifications and has been extensively characterized by proteomics and structural studies (3D structure available).
• The protein is highly conserved across mammalian species, reflected in its cross-reactivity with mouse and rat orthologs.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-160 of human RPL21, covering the full-length protein; expect native conformation recognition.
• For western blotting, a specific band near 19 kDa should be observed; validate expression in appropriate cell lines (e.g., HeLa, HEK293).
• In IHC-P and IF/ICC, optimize fixation/permeabilization to detect cytoplasmic and ER-associated RPL21.
• Cross-reactivity with mouse and rat is predicted; for other species, verify sequence similarity before use.
• For ELISA, coating and detection conditions should be optimized using recombinant or endogenous RPL21.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of validated ribosomal protein antibodies and ancillary reagents for diagnostic and research applications. With expertise in IVD raw materials, we support your RPL21-related projects from concept to clinic, including bulk supply, custom antibody development, and rare target sourcing. Let CamelBio be your one-stop bridge for high-quality ribosomal research tools.