Anti-RPL18A Polyclonal Antibody for WB and ELISA - Q02543

Core Product Specifications and Parameters

Biological Background: RPL18A Function and Localization

• RPL18A, also known as 60S ribosomal protein L18a or eL20, is a core component of the large (60S) ribosomal subunit.
• As part of the ribosome, RPL18A plays an essential role in cellular protein synthesis (translation).
• The RPL18A protein localizes primarily to the cytoplasm, where ribosomes are assembled and function.
• It is a member of the ribosomal protein L18Ae family and is classified as a ribonucleoprotein.
• RPL18A undergoes post-translational modifications including phosphorylation and ubiquitination (Ubl conjugation), as indicated by the presence of isopeptide bonds.
• The protein's 3D structure has been experimentally determined, providing insights into its interaction within the ribosomal complex.
• RPL18A is broadly expressed and is a reference proteome component, underscoring its fundamental role in cellular biology.

Experimental Guidance and Technical Tips

• The immunogen design based on amino acids 50–176 may yield antibodies that recognize full-length RPL18A and possibly isoforms; consider validating specificity in the relevant sample system.
• For Western blot, a band around 21 kDa is expected; however, actual migration may vary depending on gel conditions and post-translational modifications.
• While validated for human, mouse, and rat samples, cross-reactivity in other species should be experimentally confirmed.
• For ELISA, optimal antibody coating and detection conditions may need to be titrated; consider using a matched recombinant protein standard for quantitation.
• Due to the conserved nature of ribosomal proteins, this polyclonal antibody might detect RPL18A across a broader range of species, but this should be verified by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio enables diagnostic manufacturers and research labs to accelerate assay development by providing a comprehensive portfolio of validated IVD raw materials. This anti-RPL18A polyclonal antibody is part of our extensive offering for translational research and ribosomal biology studies. Beyond antibodies, we supply bulk ancillary reagents and offer technical support to bridge the gap from concept to clinic.