Anti-RPL18 Polyclonal Antibody for WB, ELISA - Q07020

Core Product Specifications and Parameters

Biological Background: RPL18 Function and Localization

• RPL18 is a large ribosomal subunit protein (60S ribosomal protein L18/eL18) that serves as a structural component of the ribosome, facilitating mRNA translation and protein synthesis.
  Related references: PMID:12962325, PMID:23636399, PMID:25901680
• Subcellularly, RPL18 localizes to the cytoplasm, both free in the cytosol and bound to the rough endoplasmic reticulum.
• The RPL18 gene produces multiple isoforms via alternative splicing, and the protein is post-translationally modified by phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation.
• Mutations or dysfunction in RPL18 are associated with Diamond-Blackfan anemia, a congenital disorder characterized by bone marrow failure and ribosome biogenesis defects.
• The protein is highly conserved across eukaryotes, with the antibody recognizing human, mouse, and rat forms.
• Keywords from curated databases include ribonucleoprotein, phosphoprotein, and disease variant, reflecting its functional and clinical significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to nearly full-length human RPL18 (aa 1–188), which may yield antibodies recognizing both native and denatured protein; consider this when selecting assay conditions.
• For Western blot, detect a band at approximately 22 kDa; validate with appropriate positive control lysates from human, mouse, or rat tissues or cell lines.
• For ELISA, optimize coating antigen concentration and antibody dilution empirically; the polyclonal nature provides robust signal but may require careful blocking.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop platform that connects diagnostic developers and research laboratories with high-quality IVD raw materials, including this RPL18 polyclonal antibody. In the field of ribosomal biology and protein synthesis, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Partner with us to streamline your procurement from discovery to commercialization.