Anti-RPL17 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P18621

Core Product Specifications and Parameters

Biological Background: RPL17 Function and Localization

• RPL17, also known as 60S ribosomal protein L17, L23, uL22, and PD-1, is a component of the 60S large ribosomal subunit.
• The protein is essential for protein synthesis, as part of the ribosome, a large ribonucleoprotein complex responsible for translating mRNA into proteins.
  Related references:
  PMID:12962325
  PMID:23636399
  PMID:32669547
• RPL17 belongs to the universal ribosomal protein uL22 family, conserved across prokaryotes and eukaryotes.
• Subcellularly, RPL17 is localized to the cytoplasm, consistent with its translation machinery role.
• Expression is observed in pancreas, lung, colon, cystic duct, gall bladder, kidney, and liver, with variable levels in pancreatic tumor cell lines; high in well-differentiated lines (e.g., HPAF) and lower in poorly differentiated ones (e.g., PANC-1).
• The protein is subject to alternative splicing, and mutations or dysfunction are associated with Diamond-Blackfan anemia, a ribosomopathy.

Experimental Guidance and Technical Tips

• The immunogen encompasses full-length RPL17 (amino acids 1–184), making the antibody likely to recognize endogenous protein; cross-reactivity with mouse and rat suggests conserved epitopes.
• For western blot, expect a band near 21 kDa; consider loading controls and validate the assay with known expression models.
• In immunohistochemistry, antigen retrieval conditions may need optimization for formalin-fixed paraffin-embedded tissues; pancreas or colon tissues are suitable positive controls.
• For immunofluorescence, fixation and permeabilization should be optimized to maintain ribosomal antigen integrity.
• ELISA can be employed for quantitative analysis; if developing a sandwich immunoassay, consider pairing with a capture antibody targeting a different epitope.
• As RPL17 expression can vary with cell proliferation status, normalize signals against total protein or a stably expressed ribosomal protein like RPLP0.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing hub for IVD raw materials, offering this anti-RPL17 polyclonal antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents suited for ribosomal research and oncology diagnostics. Our portfolio also includes sourcing services for rare targets and technical consultation to support your project from concept to clinic.