Anti-RPL14 Polyclonal Antibody for WB, IF/ICC, ELISA - P50914

Core Product Specifications and Parameters

Biological Background: RPL14 Function and Localization

• RPL14 is a component of the large ribosomal subunit, a ribonucleoprotein complex responsible for protein synthesis in the cell. Related references: PMID:12962325, PMID:23636399, PMID:32669547
• RPL14 localizes to the cytoplasm, where it participates in translation.
• The protein undergoes post‑translational modifications including acetylation, phosphorylation, and ubiquitination (Ubl conjugation), which may modulate ribosomal activity.
• RPL14 features a repeat region and is classified under the eL14 ribosomal protein family.
• Structural studies (3D‑structure) and direct protein sequencing have been reported, highlighting its conserved role.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 23 kDa is expected; optimize primary antibody dilution and detection conditions for your specific lysates and membrane type.
• In immunofluorescence/ICC, test fixation and permeabilization protocols to ensure adequate accessibility to the cytoplasmic RPL14 target.
• For ELISA, consider pairing this antibody with a suitable coating antigen and validate performance with recombinant RPL14 or native ribosomal extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti‑RPL14 polyclonal antibody supports research into protein synthesis and ribosomal biology. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source rare‑target raw materials to meet your assay development needs.