Anti-RPL13A Rabbit Polyclonal Antibody for WB, ELISA - P40429

Core Product Specifications and Parameters

Biological Background: RPL13A Function and Localization

• RPL13A encodes the large ribosomal subunit protein uL13 (also known as 60S ribosomal protein L13a or 23 kDa highly basic protein), a component of the 60S ribosomal subunit.
• While associated with ribosomes, uL13 is not required for canonical ribosome function and has extra-ribosomal roles, including participation in transcript-selective translation regulation. Related references: PMID:14567916, PMID:17218275, PMID:23636399
• Upon interferon-gamma stimulation, uL13 is phosphorylated and dissociates from ribosomes to assemble into the GAIT (gamma-interferon-activated inhibitor of translation) complex. This complex binds to stem-loop GAIT elements in the 3'-UTR of inflammatory mRNAs (e.g., ceruloplasmin) and suppresses their translation by blocking 43S ribosomal complex recruitment via interaction with eIF4G. Related references: PMID:23071094
• uL13 is involved in the methylation of rRNA, contributing to ribosomal RNA processing and maturation. Related references: PMID:17921318
• Post-translational modifications include acetylation, citrullination, and phosphorylation, which may regulate its extra-ribosomal functions and interaction with the GAIT complex.
• Subcellularly, uL13 localizes predominantly in the cytoplasm where it partakes in ribosomal and non-ribosomal activities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human RPL13A (aa 1-197). For Western blot, consider using whole-cell lysates from human, mouse, or rat cell lines; a specific band near 24 kDa is anticipated.
• For ELISA, the antibody can be used as a capture or detection reagent; however, titration and cross-reactivity testing with relevant controls are recommended.
• Cross-reactivity with mouse and rat suggests utility in translational models; validate expression levels in your experimental system as ribosomal protein abundance may vary with cell type and activation state.
• Given the role of RPL13A in GAIT complex formation, interferon-gamma treatment may alter its ribosome association; consider this when designing experiments involving inflammation.
• Store at -20°C or as recommended, and avoid repeated freeze-thaw cycles to maintain antibody integrity.

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CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive one-stop platform for sourcing IVD raw materials, technical services, and regulatory consulting. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for targets such as RPL13A, supporting applications from translational research to clinical assay development. With extensive supply chain capabilities, we bridge the gap between discovery and clinical application, providing reliable access to rare-target raw materials.