Anti-RPL13 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P26373

Core Product Specifications and Parameters

Biological Background: RPL13 Function and Localization

• RPL13 is a component of the large ribosomal subunit, participating in protein synthesis by contributing to the peptidyl transferase center and providing the nascent polypeptide exit tunnel. Related references: PMID:23636399, PMID:31630789, PMID:32669547
• Plays a role in the formation of the large ribosomal subunit and maturation of ribosomal RNAs, and is involved in bone development. Related references: PMID:31630789
• Also known as BBC1 (Breast Basic Conserved Protein 1) and eL13.
• Localizes primarily to the cytoplasm, consistent with its ribosomal function.
• Exhibits higher expression levels in benign breast lesions compared to carcinomas, suggesting a potential role in breast tissue differentiation.
• Subject to post-translational modifications including acetylation, phosphorylation, and isopeptide bond formation (ubiquitin-like conjugation).
• Associated with disease variants linked to dwarfism phenotypes, as indicated by UniProt keyword annotations.
• Alternative splicing generates multiple transcript variants, potentially modulating ribosomal function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human RPL13 protein (amino acids 1-211), providing broad epitope coverage. This design may enhance reactivity across species and applications.
• Polyclonal antibodies can vary between lots; consider validating each lot in your specific assay system.
• Recommended dilutions and protocols should be empirically determined for each application; start with manufacturer suggestions and optimize.
• For Western blotting, expected band size is approximately 24 kDa. Use appropriate molecular weight markers and validate with positive/negative controls.
• In IHC-P and IF/ICC, include isotype controls and perform antigen retrieval if necessary; tissue fixation can impact epitope accessibility.
• For ELISA, consider using known positive and negative samples to establish assay sensitivity and specificity.
• Cross-reactivity with mouse and rat is reported, but confirm reactivity in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This RPL13 antibody supports ribosome biology, protein synthesis studies, and cancer research applications. If your project requires rare target raw-material sourcing or customized antibody development, our team offers comprehensive procurement and technical consulting to help move your diagnostic concepts into clinical practice.