Anti-RPL11 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P62913

Core Product Specifications and Parameters

Biological Background: RPL11 Function and Localization

• RPL11 is a component of the ribosome, catalyzing peptide bond formation and facilitating nascent polypeptide chain processing.
  Related references: PMID:19191325, PMID:32669547

• As part of the 5S RNP, RPL11 is essential for large ribosomal subunit assembly and rRNA maturation.
  Related references: PMID:12962325, PMID:19061985, PMID:24120868

• RPL11 links ribosome biogenesis to p53 signaling by inhibiting MDM2 under nucleolar stress, leading to TP53 stabilization.
  Related references: PMID:24120868

• RPL11 localizes to the nucleolus and cytoplasm, and its nucleolar targeting is promoted by PML.

• Post-translational modifications of RPL11 include acetylation and phosphorylation, which may regulate its ribosomal and extra-ribosomal functions.

• Mutations in RPL11 are associated with Diamond-Blackfan anemia, and it has been identified as a CLL-associated antigen.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 1-177 amino acids of human RPL11, covering a conserved region; verify sequence homology for your target species.
• For Western blotting, the predicted molecular weight is ~20 kDa; use a 12-15% SDS-PAGE gel for optimal resolution and include appropriate molecular weight markers.
• For immunofluorescence/immunocytochemistry, optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) conditions, as RPL11 is found in both nucleolar and cytoplasmic compartments.
• In ELISA applications, titrate the antibody and consider a sandwich format with a matched detection antibody if quantifying RPL11 levels.
• Cross-reactivity with mouse and rat makes this antibody suitable for rodent model studies; nonetheless, validate performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material procurement, offering a wide range of validated antibodies and reagents to accelerate diagnostic development. For research focused on RPL11—a pivotal player in ribosome biology and p53-mediated stress pathways—CamelBio can source bulk polyclonal antibodies, monoclonal alternatives, and ancillary reagents, including rare target raw materials. With a commitment to supporting every stage from discovery to clinical application, CamelBio ensures seamless access to the critical tools you need.