Anti-RPL11 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P62913

Core Product Specifications and Parameters

Biological Background: RPL11 Function and Localization

• RPL11, also known as 60S ribosomal protein L11 or large ribosomal subunit protein uL5, is a component of the large ribosomal subunit.
• As a core ribosomal protein, RPL11 participates in protein synthesis by contributing to the peptidyl transferase center (PTC) activity, which catalyzes peptide bond formation during translation (PubMed:19191325, PubMed:32669547). Related references: PMID:19191325, PMID:32669547
• RPL11 is an essential part of the 5S ribonucleoprotein particle (5S RNP), required for large subunit formation and rRNA maturation (PubMed:12962325, PubMed:19061985, PubMed:24120868). Related references: PMID:12962325, PMID:19061985, PMID:24120868
• Beyond its structural role, RPL11 couples ribosome biogenesis to p53/TP53 activation. Upon ribosomal stress, the 5S RNP accumulates in the nucleoplasm and inhibits MDM2, leading to TP53 stabilization and activation (PubMed:24120868). Related references: PMID:24120868
• RPL11 localizes predominantly to the nucleolus and cytoplasm, and is also found in the nucleoplasm under certain conditions. It promotes nucleolar localization of PML.
• Post-translational modifications include acetylation, phosphorylation, and isopeptide bond formation. The protein is also associated with Diamond-Blackfan anemia when mutated.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-178) of RPL11. This region is relatively conserved among species, consistent with the reported cross-reactivity with human, mouse, and rat.
• For Western blot (WB), a band around 20 kDa is expected. Consider using appropriate loading controls and validate in your specific sample lysates.
• For immunofluorescence (IF/ICC), fixation and permeabilization conditions should be optimized. The antibody's performance may vary depending on cell type and endogenous expression levels.
• For ELISA, pair with a suitable detection antibody and validate with recombinant protein or cell lysates.
• Given its involvement in ribosome biogenesis stress responses, this antibody may be useful for studying p53 pathway activation under conditions that disrupt ribosome assembly.

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