Anti-RPL11 Polyclonal Antibody for IF/ICC, ELISA - P62913

Core Product Specifications and Parameters

Biological Background: RPL11 Function and Localization

• RPL11 encodes the human 60S ribosomal protein L11, also known as uL5, L11, DBA7, and GIG34. It is a core component of the large ribosomal subunit.
• As part of the ribosome, RPL11 contributes to the peptidyl transferase center (PTC) that catalyzes peptide bond formation during protein synthesis. Related references: PMID:19191325 PMID:32669547
• RPL11 is an essential component of the 5S ribonucleoprotein particle (5S RNP), required for large subunit assembly and rRNA maturation. Related references: PMID:12962325 PMID:19061985 PMID:24120868
• Under nucleolar stress, RPL11 accumulates in the nucleoplasm, where it binds and inhibits MDM2, leading to stabilization and activation of the tumor suppressor p53. Related references: PMID:24120868
• RPL11 localizes to the nucleolus and cytoplasm, dynamically shuttling in response to ribosome biogenesis status.
• The protein undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination, which may modulate its function and stability.
• Mutations in RPL11 are associated with Diamond-Blackfan anemia, a congenital bone marrow failure syndrome.
• RPL11 promotes the nucleolar localization of PML protein (By similarity).

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1–177 of human RPL11, representing the N-terminal region. This antibody may detect both full-length and truncated RPL11 species.
• For immunofluorescence (IF/ICC), consider optimizing permeabilization to visualize nucleolar and cytoplasmic RPL11 signals in human cell lines.
• In ELISA applications, empirical determination of coating antigen concentration and antibody dilution is recommended to achieve optimal signal-to-noise ratios.
• Given the high conservation of ribosomal proteins, cross-reactivity with RPL11 orthologs from other species should be validated if intended for non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our anti-RPL11 antibody supports studies in ribosome biogenesis, translational regulation, and p53-mediated stress responses. Whether you need validated antibody pairs, bulk ancillary reagents, or rare target sourcing, CamelBio offers efficient procurement solutions tailored to your project needs.