Anti-RPL10A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62906

Core Product Specifications and Parameters

Biological Background: RPL10A Function and Localization

• RPL10A encodes the 60S ribosomal protein L10a, also designated large ribosomal subunit protein uL1, and is known by alternative names including CSA-19 and NEDD-6 (neural precursor cell expressed developmentally down-regulated protein 6).
• As a core component of the large ribosomal subunit, RPL10A participates in ribosome biogenesis and protein synthesis, contributing to the translation machinery in the cytoplasm. Related references: PMID:12962325, PMID:23636399, PMID:32669547
• The RPL10A protein contains conserved ribosomal protein L1 domains, sharing homology with prokaryotic and archaeal L1 proteins, reflecting its ancient and essential role in ribosome structure.
• RPL10A is primarily localized in the cytoplasm, consistent with its role in cytosolic ribosomes, though possible nucleolar transit during ribosome assembly cannot be ruled out.
• Post-translational modifications of RPL10A include acetylation, phosphorylation, and ubiquitin-like conjugation (UBL), indicated by isopeptide bond formation, which may regulate ribosomal function or protein stability.
• Keywords such as “3D-structure”, “ribonucleoprotein”, and “Reference proteome” highlight validated structural data and its comprehensive annotation in the human proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–110) of human RPL10A; consider verifying reactivity against full-length protein and potential splice variants in your experimental system.
• For Western blot (WB), a band around 25 kDa is expected based on the calculated molecular weight; however, actual migration may vary, so always include appropriate molecular weight markers.
• This polyclonal antibody has been applied in IHC-P and IF/ICC; optimal antibody dilution should be determined by titration under your specific fixation and permeabilization conditions.
• Reported cross-reactivity with mouse and rat suggests suitability for studies in these species, but confirm reactivity in the relevant cell line or tissue lysate.
• As a rabbit polyclonal, product performance may exhibit slight lot-to-lot variation; it is advisable to validate each new lot against previous data if critical for longitudinal studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to supply high-quality IVD raw materials, including validated antibodies for ribosome biology. Beyond this RPL10A polyclonal antibody, our portfolio spans monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your development workflow.