Anti-RPL10A Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62906

Core Product Specifications and Parameters

Biological Background: RPL10A Function and Localization

• RPL10A encodes the 60S ribosomal protein L10a (also known as uL1, CSA-19, or NEDD-6), a core component of the large (60S) ribosomal subunit.
• As a structural constituent of the large ribosomal subunit, RPL10A participates in the ribonucleoprotein complex that catalyzes protein synthesis (PubMed:12962325, PubMed:23636399, PubMed:32669547). Related references: PMID:12962325, PMID:23636399, PMID:32669547
• RPL10A localizes predominantly to the cytoplasm, consistent with its role in ribosomal translation.
• Post-translational modifications of RPL10A include acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), as identified by proteomics.
• The protein belongs to the universal ribosomal protein uL1 family and is evolutionarily conserved across eukaryotes.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody spans the N-terminal 175 amino acids of human RPL10A (full-length mature protein). Consider this region when mapping epitopes or aligning across species.
• For Western blotting, the predicted molecular weight of RPL10A is approximately 25 kDa. Verify band position using a suitable molecular weight marker and consider post-translational modifications that may shift migration.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), include appropriate controls (e.g., tissue/cell lines with known RPL10A expression) to confirm specificity. Optimal dilution and antigen retrieval conditions should be determined empirically.
• This antibody is also applicable to ELISA; users should validate coating and detection conditions for their specific assay format.

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CamelBio streamlines procurement for diagnostic manufacturers and researchers by providing access to validated IVD raw materials like this RPL10A antibody, alongside bulk ancillary reagents and customized sourcing solutions. Whether you need monoclonal antibody pairs, recombinant antigens, or rare-target reagents for ribosomal and translational research, our integrated supply chain simplifies development from concept to clinic. Contact us to discuss your project requirements and scaling needs.