Anti-RPIA Polyclonal Antibody for WB, IHC-P, ELISA - P49247

Core Product Specifications and Parameters

Biological Background: RPIA Function and Localization

• RPIA, also known as ribose-5-phosphate isomerase or phosphoriboisomerase, is an enzyme encoded by the RPIA gene in humans.
• It catalyzes the reversible isomerization of ribose-5-phosphate to ribulose 5-phosphate, a key step in the non-oxidative branch of the pentose phosphate pathway.
• The pentose phosphate pathway is essential for nucleotide synthesis and NADPH generation, linking RPIA function to anabolic metabolism and redox balance.
• According to UniProt, the protein has a molecular weight of approximately 33 kDa (33269 Da).
• Mutations in RPIA are associated with disease variants including neuropathy, highlighting its clinical relevance.
• Post-translational modifications include methylation and phosphorylation, as indicated by UniProt keywords.
• The protein is classified as an isomerase and has been identified in multiple proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment of human RPIA (amino acids 82-311). Consider using the full-length recombinant protein as a positive control in applications like WB and ELISA.
• For Western blot, an expected band size of approximately 33 kDa should be observed in human, mouse, and rat samples. Optimize protein loading and antibody dilution for your specific experimental setup.
• In IHC-P, proper antigen retrieval (e.g., heat-induced epitope retrieval at pH 6 or 9) may be necessary. Validate the antibody in positive and negative tissue controls.
• Due to the polyclonal nature of this antibody, lot-to-lot variability may occur. It is advisable to include appropriate controls and consider normalization between experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, supporting projects from concept to clinic. For metabolic and nucleotide synthesis research centered on RPIA, we offer validated polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare target raw materials. Let CamelBio streamline your procurement pipeline with reliable, high-quality products.