Anti-RPH3A (Rabphilin-3A) Rabbit Polyclonal Antibody for WB, ELISA - Q9Y2J0

Core Product Specifications and Parameters

Biological Background: Rabphilin-3A Function and Localization

• Encoded by the RPH3A gene, Rabphilin-3A (also known as Exophilin-1) is a protein that undergoes alternative splicing, yielding multiple isoforms.
• Functions as a key effector for the small GTPase RAB3A, playing an essential role in the docking and fusion steps of regulated exocytosis, including neurotransmitter release and hormone secretion.
• At the presynaptic terminal, RPH3A is recruited by GTP-bound RAB3A to the synaptic vesicle membrane, where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release.
• In the postsynaptic compartment, RPH3A forms a ternary complex with GRIN2A (NMDA receptor subunit) and DLG4 (PSD-95), contributing to the stabilization of NMDA receptors at the synapse.
• Localizes to multiple membrane-associated compartments: predominantly found on the cytoplasmic side of synaptic vesicle membranes, but also detected at the postsynaptic cell membrane and within dendritic spines.
• Contains functional domains including C2 domains for calcium and lipid binding, and a zinc-finger motif, consistent with its roles in metal binding, membrane association, and calcium-dependent regulation.
• Post-translational modifications include phosphorylation, methylation, and ubiquitination, which may regulate its activity, localization, and protein interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–280) of human RPH3A; consider this region when designing complementary detection strategies or analyzing isoform-specific signals.
• For Western blot (WB) analysis, a band around 77 kDa is expected; brain tissue lysates or synaptosomal preparations may provide optimal detection due to enriched expression in synaptic compartments.
• The antibody shows cross-reactivity with mouse and rat RPH3A, enabling its use in rodent models; validation in the relevant species and sample type is recommended.
• For ELISA applications, ensure appropriate antigen coating and consider using recombinant RPH3A or native protein from tissue extracts as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and polyclonal antibodies for targets such as Rabphilin-3A. In addition to ready-to-use antibodies, we facilitate bulk procurement of ancillary reagents and can source custom or rare-target raw materials to streamline your assay development from concept to clinic.