Anti-RPF2 Rabbit Polyclonal Antibody for WB, ELISA - Q9H7B2

Core Product Specifications and Parameters

Biological Background: RPF2 Function and Localization

• RPF2 (Ribosome production factor 2 homolog), also known as Brix domain-containing protein 1 (BXDC1), is a conserved nucleolar protein essential for ribosome large subunit assembly.
• It is thought to regulate the localization of the 5S ribonucleoprotein particle (5S RNP) to the nucleolus, facilitating its integration into the pre-60S ribosomal subunit.
• The protein belongs to the Brix domain superfamily, which is characterized by a role in ribosomal RNA processing and ribosome biogenesis.
• Subcellular localization is restricted to the nucleolus, consistent with its function in early ribosome assembly steps within the nucleus.
• UniProtKB annotation classifies RPF2 under keywords including ribosome biogenesis, nucleus, and reference proteome, with experimental 3D structures available.
• No tissue-specific expression or disease associations have been characterized for this protein as of the current record.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein spanning amino acids 1–306 of human RPF2, covering nearly the full-length protein; consider this when choosing a blocking peptide or mapping epitopes.
• As a rabbit polyclonal antibody, it may recognize multiple epitopes and can be suitable for detecting denatured protein in Western blot under reducing conditions.
• Cross-reactivity is predicted with mouse and rat RPF2 based on sequence homology; validate in the relevant sample system if quantitative species comparison is required.
• For ELISA, titrate the antibody and include appropriate negative controls (e.g., pre-immune serum or unrelated antigen) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, research laboratories, and core facilities with high-quality IVD raw materials and technical services across the entire product development lifecycle. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support ribosome biogenesis and nucleolar research. Our streamlined procurement pipeline ensures reliable access to the critical reagents you need, from concept to clinic.