Anti-RPE65 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q16518

Core Product Specifications and Parameters

Biological Background: RPE65 Function and Localization

• RPE65 (retinoid isomerohydrolase), also known as all-trans-retinyl-palmitate hydrolase, lutein isomerase, and meso-zeaxanthin isomerase, is encoded by the RPE65 gene in humans (Gene ID: 6121).
• It functions as a critical isomerohydrolase in the retinoid cycle, converting all-trans-retinyl fatty acid esters to 11-cis-retinol, a vital step in regenerating the visual chromophore 11-cis-retinal.
  Related references:
  PMID:16116091
• Essential for the production of 11-cis retinal for both rod and cone photoreceptors, ensuring proper visual transduction.
  Related references:
  PMID:17848510
• Additionally, RPE65 catalyzes the isomerization of lutein to meso-zeaxanthin, an eye-specific carotenoid.
  Related references:
  PMID:28874556
• Subcellularly, RPE65 localizes to the cytoplasm, cell membrane, and microsome membrane.
• Exhibits high tissue specificity, expressed predominantly in the retinal pigment epithelium (RPE) as confirmed at the protein level in the retina.
• Post-translational modifications include palmitoylation, acetylation, and phosphorylation; the protein also binds iron as a cofactor.
• Mutations in RPE65 are linked to inherited retinal diseases such as Leber congenital amaurosis (LCA2) and retinitis pigmentosa (RP20), underscoring its critical role in vision.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 400–500 of human RPE65; consider using peptide blocking experiments to confirm specificity in your assay system.
• For Western blot (WB), expect a primary band around 61 kDa. Validate expression in appropriate retinal or RPE-derived samples.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), staining should be enriched in the retinal pigment epithelium. Include tissue-specific positive and negative controls.
• The antibody cross-reacts with mouse and rat, facilitating translation studies; however, verify reactivity under your experimental conditions.
• For ELISA, optimize coating antigen and antibody concentration based on your specific platform.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This RPE65 antibody is well-suited for vision research and retinoid cycle studies, and CamelBio can also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and facilitate sourcing of rare targets. Leverage our one-stop service to streamline your assay development and procurement needs.